Composite

Part:BBa_K2285019:Design

Designed by: Zhuoyang Chen   Group: iGEM17_SJTU-BioX-Shanghai   (2017-10-19)


J23119+Target1+amilCP(RBS+amilCP+Term)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Target1 with the downstream reporter gene is hard to PCR as a whole part for the hairpin structure.


Source

The Star1 system including Target1 and Antisense1 is originated from the AD1 strain (Chappell J, Takahashi MK, Lucks JB. 2015. Creating small transcription activating RNAs.)

References